Flow Cytometric Analysis of the Hermes Homing - Associated Antigen on Human
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چکیده
The homing of lymphocytes is controlled by interactions with high endothelial venules (HEy). specialized vessels that define sites of lymphocyte extravasation into lymph nodes and inflamed tissues. In humans. lymphocyte-HEV binding involves a lymphocyte surface glycoprotein (GP) of 85 to 95 kd (CD44. H-CAM). defined by monoclonal antibody (MoAb) Hermes-i . To define the expression of this homing-associated adhesion molecule during human lymphocyte development. we performed two-color immunofluorescence analyses of human bone marrow (BM). thymus. peripheral blood (PB). and tonsillar lymphocytes. The highest levels of Hermes-i antigen are displayed by circulating B and T cells in the blood. which are uniformly positive and bear roughly twice the level of antigen present on mature lymphocytes within organized lymphoid tissues and BM. “Immature” (CD4 . CD8I T cells in the thymus are Hermes-i #{176} . whereas thymocytes of mature phenotype (CD4 or CD8I are positive. The Hermes-i antigen is present at high levels on the same population of thymocytes that bears high surface levels of CD3. a component of
منابع مشابه
Flow cytometric analysis of the Hermes homing-associated antigen on human lymphocyte subsets.
The homing of lymphocytes is controlled by interactions with high endothelial venules (HEV), specialized vessels that define sites of lymphocyte extravasation into lymph nodes and inflamed tissues. In humans, lymphocyte-HEV binding involves a lymphocyte surface glycoprotein (GP) of 85 to 95 kd (CD44, H-CAM), defined by monoclonal antibody (MoAb) Hermes-1. To define the expression of this homing...
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